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Rapid Measurement of Methyltransferase Activity for Drug Development

Utah State University is seeking a company interested in commercializing a method that provides a simple, quick, easy way to measure the methylation of proteins in vitro. Current methods can take 1 week to 3 months to complete an analysis, but the novel method from Utah State requires only 1-2 hours. The method developed at Utah State University features an improved methyltransferase assay that utilizes simple reliable tools. This method is also more sensitive than typical testing methods, which may be used under conditions required to determine steady state kinetics and is easily adaptable to high-throughput screening formats. Additionally, because the method is not reliant on coupling enzymes the false positive rate is decreased. Radioactive waste is also decreased by 3,000 fold using this new method. Utah State researchers are finding solutions to problems in testing drug effectiveness.
Features and Benefits
  • Pharmaceuticals
  • Drug Screening
  • Academic Research
  • Extremely fast analysis (weeks/months to 1-2 hours), raising responsiveness; reducing down time
  • High sensitivity, providing more accurate analyses
  • Uses simple reliable tools, decreasing equipment costs
  • Direct measurement without the need for coupling enzymes
  • Reduction in radioactive waste, maintaining safe processes
  • Easily adapted to HTS needs
Measuring the effectiveness with which methyl groups are transferred to proteins has previously been very time-consuming, prohibiting large screens. The technology at Utah State uses reverse phase resin pipette/multi-well technology to provide an efficient replacement to these time consuming methyltransferase assays. The method also increases sensitivity by incorporating liquid scintillation counting to detect radioactivity rather than typical membrane or surface tests. This high sensitivity feature provides knowledge on steady state kinetics giving valuable information regarding pharmacokinetics. The time required for this novel method is roughly 1-2 hours whereas current technologies range from 1 week to 3 months. This method quickly and accurately measures the effectiveness of catalysts used to promote specific alterations to proteins.
Development Stage
Patent Pending
Joe Christison
Commercialization Associate
Life Sciences
(435) 797-9614
Reference: W09031